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anti myoglobin monoclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti myoglobin monoclonal antibody
    Anti Myoglobin Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myoglobin/myoglobin+Antibody/pm41855087-49-11-18
    Average 93 stars, based on 91 article reviews
    anti myoglobin monoclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: The Pierce BCA protein assay kit (Thermo Scientific) was used to quantify protein in supernatant, which was then stored at −80 °C. .. Samples were separated by SDS-PAGE, transferred onto nitrocellulose membrane, stained using Ponceau S, blocked with 5% milk in phosphate buffered saline with 0.1% Tween20 (PBST), and incubated overnight at 4 °C with primary antibodies, AMPK (#2532), p-AMPK (#2535), ERRα (# 13826), DLL4 (# 96406), and eNOS (# 9586) all at 1:1000 (Cell signaling, Danvers, MA); VEGFR2 at 1:1000 (# MA5-15157, Invitrogen, Carlsbad, CA); VEGFA (# sc-7269) at 1:250, and Myoglobin (#sc-74525) at 1:500 (Santa Cruz Biotechnology, Dallas, TX). .. Membranes were then washed with PBST and incubated with the appropriate secondary antibodies (Cell Signaling) for 1 hr. at room temperature, washed with PBST, and bands visualized using chemiluminescence western blotting detection reagents.

    Staining:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: The Pierce BCA protein assay kit (Thermo Scientific) was used to quantify protein in supernatant, which was then stored at −80 °C. .. Samples were separated by SDS-PAGE, transferred onto nitrocellulose membrane, stained using Ponceau S, blocked with 5% milk in phosphate buffered saline with 0.1% Tween20 (PBST), and incubated overnight at 4 °C with primary antibodies, AMPK (#2532), p-AMPK (#2535), ERRα (# 13826), DLL4 (# 96406), and eNOS (# 9586) all at 1:1000 (Cell signaling, Danvers, MA); VEGFR2 at 1:1000 (# MA5-15157, Invitrogen, Carlsbad, CA); VEGFA (# sc-7269) at 1:250, and Myoglobin (#sc-74525) at 1:500 (Santa Cruz Biotechnology, Dallas, TX). .. Membranes were then washed with PBST and incubated with the appropriate secondary antibodies (Cell Signaling) for 1 hr. at room temperature, washed with PBST, and bands visualized using chemiluminescence western blotting detection reagents.

    Saline:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: The Pierce BCA protein assay kit (Thermo Scientific) was used to quantify protein in supernatant, which was then stored at −80 °C. .. Samples were separated by SDS-PAGE, transferred onto nitrocellulose membrane, stained using Ponceau S, blocked with 5% milk in phosphate buffered saline with 0.1% Tween20 (PBST), and incubated overnight at 4 °C with primary antibodies, AMPK (#2532), p-AMPK (#2535), ERRα (# 13826), DLL4 (# 96406), and eNOS (# 9586) all at 1:1000 (Cell signaling, Danvers, MA); VEGFR2 at 1:1000 (# MA5-15157, Invitrogen, Carlsbad, CA); VEGFA (# sc-7269) at 1:250, and Myoglobin (#sc-74525) at 1:500 (Santa Cruz Biotechnology, Dallas, TX). .. Membranes were then washed with PBST and incubated with the appropriate secondary antibodies (Cell Signaling) for 1 hr. at room temperature, washed with PBST, and bands visualized using chemiluminescence western blotting detection reagents.

    Incubation:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: The Pierce BCA protein assay kit (Thermo Scientific) was used to quantify protein in supernatant, which was then stored at −80 °C. .. Samples were separated by SDS-PAGE, transferred onto nitrocellulose membrane, stained using Ponceau S, blocked with 5% milk in phosphate buffered saline with 0.1% Tween20 (PBST), and incubated overnight at 4 °C with primary antibodies, AMPK (#2532), p-AMPK (#2535), ERRα (# 13826), DLL4 (# 96406), and eNOS (# 9586) all at 1:1000 (Cell signaling, Danvers, MA); VEGFR2 at 1:1000 (# MA5-15157, Invitrogen, Carlsbad, CA); VEGFA (# sc-7269) at 1:250, and Myoglobin (#sc-74525) at 1:500 (Santa Cruz Biotechnology, Dallas, TX). .. Membranes were then washed with PBST and incubated with the appropriate secondary antibodies (Cell Signaling) for 1 hr. at room temperature, washed with PBST, and bands visualized using chemiluminescence western blotting detection reagents.

    Article Title: Determining muscle plasticity and meat quality development of low-input extended fed market-ready steers
    Article Snippet: Muscle proteins and controls were separated by SDS-PAGE (10%, 15%, or 18%), transferred to nitrocellulose membranes, and blocked at room temperature with either Prometheus OneBlock Blocking buffer (Genesee Scientific Corporation, El Cajon, CA) or 5% nonfat dry milk in Tris-buffered saline solution with 0.1% tween-20 (1 × TBS-T) added. .. Following blocking, membranes were incubated overnight at room temperature with primary antibodies specific for phosphofructokinase-1 (PFK, Santa Cruz Biotechnology, Inc, SC-166722 at 1:1000 dilution), calpain-1 (CAPN1; Thermo-Fisher 9A4H8D3 at 1:1000 dilution), calpastatin (CAST; Thermo-Fisher 1F7ED10 at 1:1000 dilution), citrate synthase (CS; Santa Cruz Biotechnology, Inc, SC-390693 at 1:1000 dilution), succinate dehydrogenase-a (SDH-a; Abcam ab14715 at 1:1000 dilution), lactate dehydrogenase-A (LDH; Novus NBPI48336 at 1:30 000 dilution), O-linked β-N-acetylglucosamine (O-GlcNAc; Abcam ab2739 at 1:1000 dilution), and myoglobin (Santa Cruz Biotechnology, Inc, SC-25607 at 1:1000 dilution). .. IRDye fluorescent secondary antibodies (LI-COR Biosciences, Lincoln, NE) were used for visualization of bands, and protein abundances were normalized to total protein (Revert 700 Protein Stain, Li-Cor Inc., Lincoln, NE).

    Article Title: Growth rate and finishing system alter beef color and early postmortem metabolism in Bos indicus crossbred cattle.
    Article Snippet: The aim of this study was to explore the effects of growth rate and finishing system (feedlot or pasture) on postmortem muscle metabolism and subsequent beef color development.. Seventy-two Angus × Nellore crossbred steers were randomly assigned to one of four treatment combinations: 1) feedlot, high growth rate (FH); 2) feedlot, low growth rate (FL); 3) pasture, high growth rate (PH) and 4) pasture, low growth rate (PL).. Animals were harvested either at a constant body weight (BW; 530 kg) or days on feed (DOF; 140 d).

    Western Blot:

    Article Title: Opposing gene regulatory programs governing myofiber development and maturation revealed at single nucleus resolution.
    Article Snippet: The following secondary antibody were used Alexa555 goat α-mouse IgG1 (ThermoFischer scientific, A-21127, dilution 1/1000), Alexa647 goat α-mouse IgM (ThermoFischer scientific, A-21238, dilution 1/1000). .. Western blotwas performed as described for Co-immunoprecipitation with the following antibody: Maf (Abcam, ab77071, dilution 1/1000), Vinculin (Sigma-Aldrich, V9131, dilution 1/1000), Tead4 (Abcam, ab58310, dilution 1/1000), Klf5 (Proteintech, 21017-1-AP, dilution 1/1000), Myogenin (Santa Cruz Biotechnology, sc-12732, dilution 1/1000), Parvalbumin (Millipore Sigma, MAB1572, clone name: PARV19, dilution 1/1000), Myoglobin (Santa Cruz Biotechnology, sc-7425, dilution 1/1000). .. C2C12 and HEK293T cell lines were obtained from American Type Culture Collection (CRL-1772, CRL-3216).

    Blocking Assay:

    Article Title: Determining muscle plasticity and meat quality development of low-input extended fed market-ready steers
    Article Snippet: Muscle proteins and controls were separated by SDS-PAGE (10%, 15%, or 18%), transferred to nitrocellulose membranes, and blocked at room temperature with either Prometheus OneBlock Blocking buffer (Genesee Scientific Corporation, El Cajon, CA) or 5% nonfat dry milk in Tris-buffered saline solution with 0.1% tween-20 (1 × TBS-T) added. .. Following blocking, membranes were incubated overnight at room temperature with primary antibodies specific for phosphofructokinase-1 (PFK, Santa Cruz Biotechnology, Inc, SC-166722 at 1:1000 dilution), calpain-1 (CAPN1; Thermo-Fisher 9A4H8D3 at 1:1000 dilution), calpastatin (CAST; Thermo-Fisher 1F7ED10 at 1:1000 dilution), citrate synthase (CS; Santa Cruz Biotechnology, Inc, SC-390693 at 1:1000 dilution), succinate dehydrogenase-a (SDH-a; Abcam ab14715 at 1:1000 dilution), lactate dehydrogenase-A (LDH; Novus NBPI48336 at 1:30 000 dilution), O-linked β-N-acetylglucosamine (O-GlcNAc; Abcam ab2739 at 1:1000 dilution), and myoglobin (Santa Cruz Biotechnology, Inc, SC-25607 at 1:1000 dilution). .. IRDye fluorescent secondary antibodies (LI-COR Biosciences, Lincoln, NE) were used for visualization of bands, and protein abundances were normalized to total protein (Revert 700 Protein Stain, Li-Cor Inc., Lincoln, NE).

    Article Title: Growth rate and finishing system alter beef color and early postmortem metabolism in Bos indicus crossbred cattle.
    Article Snippet: The aim of this study was to explore the effects of growth rate and finishing system (feedlot or pasture) on postmortem muscle metabolism and subsequent beef color development.. Seventy-two Angus × Nellore crossbred steers were randomly assigned to one of four treatment combinations: 1) feedlot, high growth rate (FH); 2) feedlot, low growth rate (FL); 3) pasture, high growth rate (PH) and 4) pasture, low growth rate (PL).. Animals were harvested either at a constant body weight (BW; 530 kg) or days on feed (DOF; 140 d).

    Pyrolysis Gas Chromatography:

    Article Title: Improved skeletal muscle mass and strength through Protamex-mediated hydrolysis of perilla seed cake: Elevated rosmarinic acid levels as a contributing factor.
    Article Snippet: Perilla seed cake (PSC) is a byproduct of oil extraction from perilla seeds.. It is rich in proteins and bioactive compounds.. PSC was enzymatically hydrolyzed to form PSC hydrolysate (PSCH) to enhance the absorption of PSC, and their effects on muscle health in mice were compared.

    Article Title: FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment
    Article Snippet: Antibodies directed against PE-anti-CD31 (553373, 1:200 dilution) and CD31 (550274, 1:200 dilution) were from BD Biosciences. .. Antibodies directed against mPDGFRβ (AF1042, 1:40 dilution) were from R&D Systems, antibodies directed against Brilliant Violet 421-anti-F4/80 (123132, 1:200 dilution), Alexa Fluor 647-anti-CD206 (141712, 1:200 dilution), FITC-anti-CD80 (104705, 1:200 dilution) and APC/Cyanine 7-anti-CD45 (103116, 1:200 dilution) were from Biolegend, antibodies directed against Ki67 (ab15580, 1:500 dilution) was from Abcam, antibodies directed against α-tubulin (bs1699, 1:5000 dilution) were from Bioworld; antibodies directed against NG2 (55027-1-AP, 1:200 dilution), CDH5 (27956-1-AP, 1:1000 dilution) and Hsp90 (13171-1-AP, 1:1000 dilution) were from Proteintech; antibodies directed against Myoglobin (sc-25607, 1:1000 dilution) were from Santa Cruz Biotechnology; antibodies directed against PGC-1α (1:1000 dilution) was developed in the laboratory of Daniel Kelly as described previously ; anti-FNIP1 was developed in the laboratory of Zhenji Gan with the help with Abcam (ab236547, 1:500 dilution). .. The secondary antibody Alexa Fluor 488 (A-11006, 1:400 dilution), Alexa Fluor 594 (A-21209, 1:400 dilution), Alexa Fluor 568 (A-11011, 1:400 dilution) was from Invitrogen; Alexa Fluor 488 (abs20026, 1:400 dilution) was from Absin.

    Article Title: FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment.
    Article Snippet: Antibodies directed against PE-anti-CD31 (553373, 1:200 dilution) and CD31 (550274, 1:200 dilution) were from BD Biosciences. .. Antibodies directed against mPDGFRβ (AF1042, 1:40 dilution) were from R&D Systems, antibodies directed against Brilliant Violet 421-anti-F4/80 (123132, 1:200 dilution), Alexa Fluor 647-anti-CD206 (141712, 1:200 dilution), FITC-anti-CD80 (104705, 1:200 dilution) and APC/Cyanine 7- anti-CD45 (103116, 1:200 dilution) were from Biolegend, antibodies directed against Ki67 (ab15580, 1:500 dilution) was from Abcam, antibodies directed against α-tubulin (bs1699, 1:5000 dilution) were from Bioworld; antibodies directed against NG2 (55027-1-AP, 1:200 dilution), CDH5 (27956-1-AP, 1:1000 dilution) and Hsp90 (13171-1-AP, 1:1000 dilution) were from Proteintech; antibodies directed against Myoglobin (sc-25607, 1:1000 dilution) were from Santa Cruz Biotechnology; antibodies directed against PGC-1α (1:1000 dilution) was developed in the laboratory of Daniel Kelly as described previously66; anti-FNIP1 was developed in the laboratory of Zhenji Ganwith the help with Abcam (ab236547, 1:500 dilution). .. The secondary antibody Alexa Fluor 488 (A-11006, 1:400 dilution), Alexa Fluor 594 (A-21209, 1:400 dilution), Alexa Fluor 568 (A-11011, 1:400dilution)was from Invitrogen; Alexa Fluor 488 (abs20026, 1:400 dilution) was from Absin.



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    Image Search Results


    ( A ) Immunoblot of nuclear c-MYC and Neurogenin-2 (NGN2) in unedited patient 1 NPCs treated with DMSO or 20 μM nocodazole (Noc) for 7 days. TBP was used as a loading control. ( B ) Quantification of neurite lengths in patient 1 NPCs treated with DMSO or 20 μM Noc at the NPC stage and throughout 14 day neuronal differentiation. ( C ) Immunoblot of nuclear c-MYC and Neurogenin-2 in patient 1 NPCs transduced with lentivirus expressing control shRNA (shSCR) or shMYC. ( D ) Quantification of neurite lengths in patient 1 NPCs transduced with shSCR or shMYC. The dashed line denotes the mean neurite length in corrected neurons. ( E ) Representative recordings of intracellular Ca 2+ dynamics in neurons transduced with shSCR ( n = 29) or shMYC ( n = 24), captured every 10 seconds using Fura-2 ratio imaging during 45 mM KCl application. Neurons were differentiated for 47 days. ( F ) Immunoblot of nuclear c-MYC and Neurogenin-2 in patient 1 NPCs treated with DMSO or 50 μM EN4 for the indicated durations. ( G ) Quantification of neurite lengths in patient 1 NPCs treated with DMSO, EN4 throughout differentiation, EN4 at the NPC stage and throughout differentiation, or EN4 only at the NPC stage. The dashed line indicates the corrected neuron mean. ( H ) Representative Ca 2+ recordings in neurons treated with DMSO ( n = 28) or 50 μM EN4 ( n = 26), acquired as in E . For B , D , and G , neurite lengths were measured using the SNT plug-in in ImageJ. Data are shown as mean ± 1 SEM. Significance was determined by unpaired 2-tailed Student’s t test for B and D or 1-way ANOVA with Tukey’s HSD test for G .

    Journal: The Journal of Clinical Investigation

    Article Title: l -2-Hydroxyglutarate impairs neuronal differentiation through epigenetic activation of MYC expression

    doi: 10.1172/JCI197010

    Figure Lengend Snippet: ( A ) Immunoblot of nuclear c-MYC and Neurogenin-2 (NGN2) in unedited patient 1 NPCs treated with DMSO or 20 μM nocodazole (Noc) for 7 days. TBP was used as a loading control. ( B ) Quantification of neurite lengths in patient 1 NPCs treated with DMSO or 20 μM Noc at the NPC stage and throughout 14 day neuronal differentiation. ( C ) Immunoblot of nuclear c-MYC and Neurogenin-2 in patient 1 NPCs transduced with lentivirus expressing control shRNA (shSCR) or shMYC. ( D ) Quantification of neurite lengths in patient 1 NPCs transduced with shSCR or shMYC. The dashed line denotes the mean neurite length in corrected neurons. ( E ) Representative recordings of intracellular Ca 2+ dynamics in neurons transduced with shSCR ( n = 29) or shMYC ( n = 24), captured every 10 seconds using Fura-2 ratio imaging during 45 mM KCl application. Neurons were differentiated for 47 days. ( F ) Immunoblot of nuclear c-MYC and Neurogenin-2 in patient 1 NPCs treated with DMSO or 50 μM EN4 for the indicated durations. ( G ) Quantification of neurite lengths in patient 1 NPCs treated with DMSO, EN4 throughout differentiation, EN4 at the NPC stage and throughout differentiation, or EN4 only at the NPC stage. The dashed line indicates the corrected neuron mean. ( H ) Representative Ca 2+ recordings in neurons treated with DMSO ( n = 28) or 50 μM EN4 ( n = 26), acquired as in E . For B , D , and G , neurite lengths were measured using the SNT plug-in in ImageJ. Data are shown as mean ± 1 SEM. Significance was determined by unpaired 2-tailed Student’s t test for B and D or 1-way ANOVA with Tukey’s HSD test for G .

    Article Snippet: MYC knockdown was performed using lentiviral shRNA constructs targeting human MYC (OriGene), with a nontargeting shRNA (shSCR) as control.

    Techniques: Western Blot, Control, Transduction, Expressing, shRNA, Imaging